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eChemShop - Chemical Reagents, Natural Compounds, Software, HPLC columns :: dPEG™ Reagents :: dPEG™ Biotinylation Reagents and Dye Label :: NHS-biotin

NHS-biotin

Product Features:

- Amine reactive label
- Critical and versatile building block for many biotinylation reagents
- Quanta’s exclusive synthetic process produces the highest quality commercial material
- Efficient label for certain peptides and nucleic acids
- Permeates cell membranes
- ULTRA PURE

General Biotin/Biotinylation References:

- Wilchek and Bayer: “Avidin-Biotin Technology”, Methods in Enzymology, Vol. 184, M.
Wilchek and E. A. Bayer, eds., 1990, Academic Press, NY.
- Hermanson: Greg T. Hermanson, “Bioconjugate Techniques”, Academic Press, Inc.,
San Diego, CA, 1996 (ISBN 0-12-342335-X). Note: This reference is an extremely
practical and informative volume from an experienced practitioner.

Protocol:

Hermanson, pg. 377, except make a 40 mg/ml solution of the NHS-biotin in dry DMF.
The use of dry DMF is critical. This can easily be obtained by placing 3 Angstrom or 4
Angstrom molecular sieves into reagent grade DMF, shaking and letting stand at least
overnight. If possible handle the NHS-biotin under an inert atmosphere. We find that
handling these materials in an Atmosbag, available from Sigma-Aldrich, under argon
(preferred) or nitrogen, is the best, most convenient and inexpensive means of handling
these moisture sensitive materials. The remaining solid NHS-biotin should be stored
under an inert gas (argon preferred) at about 4oC.

Details


SKU	BQ-10205-100
Weight	0.00 lbs
Price:	$65.00 (€ 51.16)

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