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eChemShop - Chemical Reagents, Natural Compounds, Software, HPLC columns :: Protease Inhibitor Cocktail
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Protease Inhibitor Cocktail |
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| Protease Inhibitor Cocktail (EDTA free) |
| Inhibition of intra and extra cellular proteases is vital to purify and collect the expressed proteins. Saving trouble of finding adequate inhibitors, a wide range of proteases are inhibited by the Protease Inhibitor Cocktail. This product does not interfere with metalloproteins since it is EDTA free. |
| The product does not contain phosphorylated proteins.The product is phosphatase free.The preservative in the product does not affect the enzyme activity of horseradish peroxidase (HRP).Store the product in refrigerator after opening. |
Wide range: Contains inhibitors for a variety of serin and cysteine proteases
Contain no EDTA: efficient for purification of metalloproteins and His tag gene exchanged proteins
Easy to use: dilute according to your need (100X stock concentration) |
| Application |
Enzyme inhibition efficiency
| Origin of Protease |
Protease |
Protease Inhibitor Cocktail (EDTA free)
(Protease concentration) |
Papain
(from papaya) |
Cystein |
100%
(2.5 µg/ml) |
96.1%
(5.0 µg/ml) |
96.5%
(20 µg/ml) |
Trypsin
(from porcine pancreas) |
Serine |
96.9%
(2.0 µg/ml) |
94.0%
(4.0 µg/ml) |
88.1%
(8.0 µg/ml) |
Chymotrypsin
(from bovine pancreas) |
Serine |
95.1%
(0.75 µg/ml) |
90.5%
(1.5 µg/ml) |
83.1%
(3.0 µg/ml) |
Pronase
(from Streptomyces griseus) |
Mixture |
96.5%
(0.6 µg/ml) |
95.3%
(1.2 µg/ml) |
92.3%
(2.4 µg/ml) |
Pancreas extract
(bovine) |
Mixture |
89.8%
(5.0 µg/ml) |
87.2%
(10 µg/ml) |
85.6%
(20 µg/ml) |
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| Composition |
The product contains:
| Inhibitors |
Targeted proteasee |
| 50 mmol/l 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) |
Serine protease |
| 15 µmol/l Aprotinin |
Serine protease, elastase |
| 100 µmol/l E-64 |
Cystein protease |
| 100 µmol/l Leupeptin hemisulfate monohydrate |
Cystein protease, trypsin like protease |
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| Protocol |
Dissolve the powdered product by adding 1 ml deionized water into the bottle to make the 100 times concentrated stock solution.Dilute with your choice of buffer to make 100 ml of 1X protease inhibitor solution. |
| Caution |
In case of very high protease concentration, use higher concentration of the product (dilute only up to between 50 and 20 times).Keep the inhibitor cocktail stock solution at -20? or lower, and use it within 3 months. |
| Expiration |
For powder in unopened bottle: two years from manufacturing.
Expiration date is stated on the product label (Exp. yy / mm) |
| Protease Inhibitor Cocktail for General Use (100X) |
| Features |
The product contain 5 kinds of protease inhibitors for different targets.The product is 100 times concentrated stock solution. Dilute with any buffer of your choice. |
| Composition |
The product contains:
| Inhibitors
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Targeted proteasee
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| 50 mmol/l 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF)
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Serine protease
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| 15 µmol/l Aprotinin from bovine lung
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Serine protease, elastase
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| 100 µmol/l E-64
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Cystein protease
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| 100 µmol/l Leupeptin hemisulfate monohydrate
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Cystein protease, trypsin like protease
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| 50 µmol/l Disodium dihydrogen ethylenediaminetetraacetate dihydrate
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Metallo protease
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| Protocol |
Dissolve the powdered product by adding 1 ml deionized water into the bottle to make the 100 times concentrated stock solution.Dilute with your choice of buffer to make 100 ml of 1X protease inhibitor solution. |
| Caution |
In case of very high protease concentration, use higher concentration of the product (dilute only up to between 50 and 20 times).Keep the inhibitor cocktail stock solution at -20? or more, and use it within 3 months. |
| Expiration |
For powder in unopened bottle: two years from manufacturing. Expiration date is stated on the product label (Exp. yy / mm) |
| Protease Inhibitor Cocktail for Use with Mammalian Cell and Tissue Extracts |
| Features |
The product contain 6 kinds of protease inhibitors for different targets.The product is a concentrated type. Dilute with any buffer of your choice. |
| Composition |
The protease inhibitors are mixed in Dimethyl sulfoxide (DMSO)
| Inhibitors |
Targeted proteasee |
| 100 mmol/l 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) |
Serine protease |
| 80 µmol/l Aprotinin from bovine lung |
Serine protease, elastase |
| 1.5 mmol/l E-64 |
Cystein protease |
| 2 mmol/l Leupeptin hemisulfate monohydrate |
Cystein protease, trypsin like protease |
| 5 mmol/l Bestatin |
Aminopeptidase |
| 1 mmol/l Pepstatin A |
Asparticprotease |
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| Protocol |
Dilute 50 - 100 times with any buffer of your choice |
| Caution |
In case of very high protease concentration, use higher concentration of the product |
| Expiration |
One year from manufacturing. Expiration date is stated on the product label (Exp. yy / mm) |
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