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COSMOSIL Sugar-D*NEW*
Conventionally Aminopropyl bounded stationary phases are used for liquid chromatographic analysis
of mono- and oligosaccharides. General shortcomings of the conventional Aminopropyl bounded
phases are tailing and adsorption of certain saccharides and general low durability (short active life)
of these columns. These problems are addressed and solved by the novel COSMOSIL Sugar-D,
resulting in better (sharper) separation and much improved durability. In addition COSMOSIL Sugar-D
is useful in the separation of highly hydrophilic compounds which are not retained in conventional
octadecyl (ODS) bounded stationary phases.
Durability :
The decrease of retention time was compared between COSMOSIL Sugar-D and conventional
Aminopropyl bounded stationary phase under severe condition of 100% water as eluent between tests.
The capacity factor did not decrease in case of COSMOSIL Sugar-D.
| Eluent condition |
Eluent
Flow rate
Temperature |
: water
: 1.0 ml/min
: RT |
| Test condition |
Column size
Mobile phase
Flow rate
Temperature
Detection
Sample |
: 4.6 mmI.D.×250 mm
: acetonitrile:water=70:30
: 1.0 ml/min
: 30°C
: RI
: Maltose |
Absorption characteristic :
Certain types of saccharides such as Arabinose or Galactose are partially or temporarily absorbed
in conventional Aminopropyl stationary phases causing disappearance of sample or tailing. These
problematic saccharides elute sharply and in proportion of the injection volume from the superior
COSMOSIL Sugar-D.
Chromatogram :
Analitical curve :
| Condition |
Column size
Mobile phase
Flow rate
Temperature
Detection
Sample |
: 4.6 mmI.D.×250 mm
: acetonitrile:water=70:30
: 1.0 ml/min
: 30°C
: RI
1.Arabinose
2.Galactose
3.Maltitol |
Application
Retention time
The conventional Aminopropyl column is slightly more retentive than Sugar-D. The retention
time can be adjusted by increasing the concentration of acetonitrile in the mobile phase by 5% - 10%.
Column size 4.6mmI.D.×250mm Flow rate 1.0ml/min Temperature: 30°C Detection: RI Sample 1: Glucose 2: Maltose
Separation of Saccharides
Mono- and Oligosaccharides Polyols
Oligomaltoses
Acidic Sugar
Cylclodextrin
Amino Sugar
The separation performances and the adsorption of monosaccharides were compared in COSMOSIL
Sugar-D and other companies' columns. The presence of Aldehyde-group in the sample saccharides
(except of Fructose) does not result in undesirable adsorption, therefore has no ill effect on the
performance of COSMOSIL Sugar-D column.
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