Price per Unit (piece):
$175.00
(including 0 % tax)
Attributes
Reactivity 1
carbonyl
Reactivity 2
sulfhydryl
Spacer length (Å)
21.1
Spacer Length (atoms)
18
Molecular Weight
513.58
Special Notes
The general application of this class of heterobifunctional crosslinkers is the controlled and selective conjugation of an amine-containing target (reacts with the NHS ester), with a sulfhydryl containing complementary target molecule (reacts with the maleimide). Many biological molecules contain the amine function or can be designed to place the thiol at a specific site AND the complementary compounds, like peptides, oligos or other biologicals can be terminated with thiols or have thiols designed into them, and vise versa. The conjugation is normally carried out in this order. Specific examples of the use of the NHS-dPEG™-MAL heterobifunctionals include Hapten-Carrier conjugation, Enzyme-antibody conjugation and protein-Avidin/Streptavidin conjugation.
Protocols
Hermanson, Greg T, Bioconjugate Techniques, Academic Press, Inc., San Diego, CA, 1996 (ISBN 0-12-342335-X). Use protocol on pages 228-248. Specifically, the sample protocol for sulfo-SMCC on pages 236-237. For the present product, it is probably still best to use an organic solvent like DMAC, DMF, or DMSO to ensure complete solubilization of the crosslinker. If water or buffer is preferred, use the standard precautions related to the stabilities of both NHS ester and maleimide.
References
See Greg T. Hermanson, Bioconjugate Techniques, Academic Press, Inc., San Diego, CA, 1996 (ISBN 0-12-342335-X), See pp. 228-248 for general description and use, and the specific sample protocol for sulfo-SMCC on pp. 236-237. Specific application protocols include a) Hapten-Carrier conjugation, pp. 439-446 b) Enzyme antibody conjugation, pp. 461-465 c) protein-Avidin/Streptavidin, pp. 575-580. For the present products it is probably still best to use an organic solvent like DMAC, DMF or DMSO to ensure complete solubilization and homogeneity of the crosslinker in the crosslinking buffer system. If water or buffer is preferred, use the standard precautions related to the stabilities of both NHS ester and maleimide, by maintaining an acid pH.
3-(3,4-dimethoxyphenyl)pyrazole-4-carbaldehyde
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dPEG Reagents 
